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Part:BBa_K2872881

Designed by: Dina Altarawneh   Group: iGEM18_CMUQ   (2018-10-10)


Template DNA sequence coding for HBB rs334 mutation (A to T) in humans, PAM site added

This is the activating sequence of Cas12a. Upon gRNA-directed binding of Cas12a to this sequence, there is an indiscriminate degradation of DNA by Cas12a. Sequence information was first obtained using OMIM: https://www.omim.org/entry/141900?search=rs334&highlight=rs334


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 178


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